What does it consist of?
It is a technique that allows a couple of women to be mothers, so that both participate actively in the reproductive process.
The ROPA Method (Reception of oocytes from the couple) consists of an in vitro fertilization using the eggs of one of them and transferring the embryo to the other. This way it is a partnership, in which one contributes their reproductive cell and the other their uterus for the development of gestation, which can be said to be the maximum expression of what we call a “shared maternity”.
For whom is it indicated?
- Couples of women who want to share the reproductive process
- Medical contraindication of pregnancy in one of them.
Control of folicular development
During the treatment of ovarian stimulation, we will perform regular controls, by vaginal ultrasound and if necessary, hormonal analysis; all this to check follicular maturation and schedule the ideal time for puncture. This phase lasts between 10 and 12 days.
The removal of the eggs is a very simple procedure that is performed in the operating room, by means of the vaginal puncture guided by ultrasound and the aspiration of the follicles. The process does not last more than 15 minutes and is performed under sedation for your comfort and well-being.
It does not require hospital admission, only a rest of several hours is prescribed in the room of our clinic. After this time, you can resume your usual activity.
Preparation of the seminal sample
The choice of the sperm donor will be made according to the phenotypic criteria of the pregnant woman.
It is necessary to prepare the semen so that it has the ability to fertilize the egg, in addition to achieving an optimal concentration of mobile sperm.
In this case the use of donor semen is indicated, this sample will always be frozen and stored in our semen bank. Our biologists will treat it in the laboratory with a process known as sperm training, so that we can select the sperm with the best mobility.
Fertilization and culture
Our embryologists perform fertilization through a technique called ICSI (Intracytoplasmic sperm injection). It consists of the selection of a single sperm for each egg that is microinjected into it. It is a very precise technique that favors the fusion of the nuclei of the male gamete with the female. Fertilized embryos must be kept in culture for 5-7 days. And during this time, they will develop until they reach the blastocyst stage.
In Next Fertility, we use time-lapse technology, which allows real-time monitoring of the evolution of embryonic development. In this way, we evaluate in a very exhaustive way the morpho-kinetic changes that are produced and this allows us to select embryos most likely to get pregnant.
The objective of this phase is to prepare the endometrium of the woman who will receive the embryo to maximize the possibilities of implantation. It is performed with the administration of estrogen and progesterone preparations, which contribute to the correct development of the endometrium, which is the layer of the uterus where the embryo will implant and the future baby will develop during its intrauterine life.
During this phase ultrasound checks and hormonal determinations are made to check the correct preparation of the endometrium.
In the embryo transfer we will deposit the embryo inside the uterine cavity of the woman who will carry out the gestation; this can be done in “fresh” or in “deferred”.
The transfer is done with an embryo in blastocyst stage (5-7 days of embryonic life), is called in “fresh” when it is done with a blastocyst without having been frozen previously, during the same cycle of ovarian stimulation and follicular puncture; and in “delayed” when a thawed blastocyst is transferred, in a cycle after the in vitro fertilisation treatment cycle.
The transfer is made “deferred” when: a) there is a risk of “Ovarian hyperstimulation syndrome”, b) endometrial conditions are not adequate for embryonic implantation, c) it is necessary to perform embryonic genetic diagnosis techniques, d) there is some reason why gestation in the same IVF cycle is not advisable. In both cases, the transfer is done with a transfer tube or catheter, using an ultrasound guide. This procedure is completely painless and does not require hospital admission
After the “beta-waiting” period (14-15 days after insemination), a blood pregnancy test will be done. We will look for the presence of the hormone β-hCG (human chorionic gonadotropin), which is the hormone produced by the embryo after its implantation in the endometrium.
If the beta test was positive, two weeks later we will perform an ultrasound, to confirm the presence of “gestational sac” inside the uterus and observe the heartbeat of your future baby.
Do you have more questions? We will be delighted to assist you.
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